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cfx96 real time pcr detection platform  (Bio-Rad)


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    Structured Review

    Bio-Rad cfx96 real time pcr detection platform
    Cfx96 Real Time Pcr Detection Platform, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15056 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx384+real+time+system/CFX96+and+CFX384+Real-Time+PCR+Detection+Systems+Firmware+Update/pmc12963904-146-10-15
    Average 99 stars, based on 15056 article reviews
    cfx96 real time pcr detection platform - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Early salt shock uncouples shoot - root acclimation in Lobularia maritima
    Article Snippet: The cDNA was used as template for Reverse-Transcriptase quantitative real-time PCR (RT-qPCR) using iTaqTM Universal SYBR Green Supermix (Bio-Rad, California, United States). .. Detection of fluorescence emission was performed on a CFX384 Real-Time System (Bio-Rad, California, United States). ..

    Polymerase Chain Reaction:

    Article Title: Characterization of the Composition and Immunoregulatory Activity of Wheat Cell Culture-Derived Polysaccharides.
    Article Snippet: .. Real-time monitoring of PCR amplification was performed using the CFX384 Real-Time System (Bio-Rad). ..

    Article Title: Redox-dependent extracellular interaction networks of Cysteine-rich Receptor-Like Kinases
    Article Snippet: DNase treatment was performed using the RNase-Free DNase Set (Qiagen) prior to cDNA synthesis, which was achieved with the iScript Select cDNA Synthesis Kit (Bio-Rad). .. Finally, relative expression levels were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using iQ SYBR Green Supermix with the CFX384 Real-Time System (Bio-Rad). ..

    Amplification:

    Article Title: Characterization of the Composition and Immunoregulatory Activity of Wheat Cell Culture-Derived Polysaccharides.
    Article Snippet: .. Real-time monitoring of PCR amplification was performed using the CFX384 Real-Time System (Bio-Rad). ..

    Quantitative RT-PCR:

    Article Title: Loss of KMT2D accelerates hypertrophic chondrocyte differentiation and senescence by increasing mitochondrial ROS production
    Article Snippet: The following primers were used: Hif1a primers (IDT, Mm.PT.58.11211292), Col10a1_F (5’-CATAAAGGGCCCACTTGCTA-3’), Col10a1_R (5’-CAGGAATGCCTTGTTCTCCT-3’), Hprt_F (5’-TCAGTCAACGGGGGACATAAA-3’), Hprt_R (5’-GGGGCTGTACTGCTTAACCAG-3’), Sox9_F (5’- CGGAACAGACTCACATCTCTCC-3’), Sox9_R (5’-GCTTGCACGTCGGTTTTGG-3’), Col2a1_F (5’- CGGTCCTACGGTGTCAGG-3’), Col2a1_R (5’- GCAGAGGACATTCCCAGTGT-3’). .. RT-qPCR was performed on a CFX384 Real-Time System (Bio-Rad), and analysis performed with CFXmanager software (Bio-Rad, v. 3.1). .. For the assessment of cellular respiration, XF Cell Mito Stress test was performed using a Seahorse XFe96 FluxPaks (Seahorse Bioscience, 102416-100) according to manufacturer’s protocol.

    Article Title: Distinct Molecular Responses to Ketamine and Imipramine in Cortical and Striatal Regions Following Acute Swim Stress
    Article Snippet: .. RNA concentration and purity were determined using a NanoDrop spectrophotometer (Thermo Fisher, Wilmington, DE, USA), and samples were diluted to 10 ng/μL for qRT-PCR. qRT-PCR was performed on a CFX384 real-time system (Bio-Rad Laboratories, Segrate, Italy) using the iScriptTM One-Step RT-PCR Kit for Probes (Bio-Rad Laboratories, Segrate, Italy). ..

    Software:

    Article Title: Loss of KMT2D accelerates hypertrophic chondrocyte differentiation and senescence by increasing mitochondrial ROS production
    Article Snippet: The following primers were used: Hif1a primers (IDT, Mm.PT.58.11211292), Col10a1_F (5’-CATAAAGGGCCCACTTGCTA-3’), Col10a1_R (5’-CAGGAATGCCTTGTTCTCCT-3’), Hprt_F (5’-TCAGTCAACGGGGGACATAAA-3’), Hprt_R (5’-GGGGCTGTACTGCTTAACCAG-3’), Sox9_F (5’- CGGAACAGACTCACATCTCTCC-3’), Sox9_R (5’-GCTTGCACGTCGGTTTTGG-3’), Col2a1_F (5’- CGGTCCTACGGTGTCAGG-3’), Col2a1_R (5’- GCAGAGGACATTCCCAGTGT-3’). .. RT-qPCR was performed on a CFX384 Real-Time System (Bio-Rad), and analysis performed with CFXmanager software (Bio-Rad, v. 3.1). .. For the assessment of cellular respiration, XF Cell Mito Stress test was performed using a Seahorse XFe96 FluxPaks (Seahorse Bioscience, 102416-100) according to manufacturer’s protocol.

    Expressing:

    Article Title: Redox-dependent extracellular interaction networks of Cysteine-rich Receptor-Like Kinases
    Article Snippet: DNase treatment was performed using the RNase-Free DNase Set (Qiagen) prior to cDNA synthesis, which was achieved with the iScript Select cDNA Synthesis Kit (Bio-Rad). .. Finally, relative expression levels were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using iQ SYBR Green Supermix with the CFX384 Real-Time System (Bio-Rad). ..

    Reverse Transcription:

    Article Title: Redox-dependent extracellular interaction networks of Cysteine-rich Receptor-Like Kinases
    Article Snippet: DNase treatment was performed using the RNase-Free DNase Set (Qiagen) prior to cDNA synthesis, which was achieved with the iScript Select cDNA Synthesis Kit (Bio-Rad). .. Finally, relative expression levels were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using iQ SYBR Green Supermix with the CFX384 Real-Time System (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Redox-dependent extracellular interaction networks of Cysteine-rich Receptor-Like Kinases
    Article Snippet: DNase treatment was performed using the RNase-Free DNase Set (Qiagen) prior to cDNA synthesis, which was achieved with the iScript Select cDNA Synthesis Kit (Bio-Rad). .. Finally, relative expression levels were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using iQ SYBR Green Supermix with the CFX384 Real-Time System (Bio-Rad). ..

    Article Title: Liver steatosis induced by per- and polyfluoroalkyl substances exerts a limited influence on heterocyclic aromatic amine-mediated DNA damage at population-relevant exposure levels.
    Article Snippet: RT-qPCR analysis 202 cDNA was synthesized from extracted RNA using the High-Capacity cDNA Reverse Transcription Kit 203 (Applied Biosystems, Thermo Fischer Scientific, Waltham, MA, USA). .. Quantitative PCR was carried out, on 1 204 ng equivalent mRNA, using the CFX384 Real-Time System (Bio-Rad, Hercules, CA, USA) with SYBR Green 205 chemistry (Applied Biosystems, Thermo Fischer Scientific), following the manufacturer’s instructions. ..

    Concentration Assay:

    Article Title: Distinct Molecular Responses to Ketamine and Imipramine in Cortical and Striatal Regions Following Acute Swim Stress
    Article Snippet: .. RNA concentration and purity were determined using a NanoDrop spectrophotometer (Thermo Fisher, Wilmington, DE, USA), and samples were diluted to 10 ng/μL for qRT-PCR. qRT-PCR was performed on a CFX384 real-time system (Bio-Rad Laboratories, Segrate, Italy) using the iScriptTM One-Step RT-PCR Kit for Probes (Bio-Rad Laboratories, Segrate, Italy). ..

    Spectrophotometry:

    Article Title: Distinct Molecular Responses to Ketamine and Imipramine in Cortical and Striatal Regions Following Acute Swim Stress
    Article Snippet: .. RNA concentration and purity were determined using a NanoDrop spectrophotometer (Thermo Fisher, Wilmington, DE, USA), and samples were diluted to 10 ng/μL for qRT-PCR. qRT-PCR was performed on a CFX384 real-time system (Bio-Rad Laboratories, Segrate, Italy) using the iScriptTM One-Step RT-PCR Kit for Probes (Bio-Rad Laboratories, Segrate, Italy). ..

    One Step RT-PCR:

    Article Title: Distinct Molecular Responses to Ketamine and Imipramine in Cortical and Striatal Regions Following Acute Swim Stress
    Article Snippet: .. RNA concentration and purity were determined using a NanoDrop spectrophotometer (Thermo Fisher, Wilmington, DE, USA), and samples were diluted to 10 ng/μL for qRT-PCR. qRT-PCR was performed on a CFX384 real-time system (Bio-Rad Laboratories, Segrate, Italy) using the iScriptTM One-Step RT-PCR Kit for Probes (Bio-Rad Laboratories, Segrate, Italy). ..

    Real-time Polymerase Chain Reaction:

    Article Title: SARS-CoV-2-infected cardiomyocytes exhibit upregulated necroptosis, but no evidence of mitochondrial permeability transition
    Article Snippet: RNA (500 ng) was reverse transcribed according to the manufacturer's instructions using the Takara PrimeScript RT Master Mix (#RR036A). .. A SYBR Green-based real-time PCR (40 cycles, Annealing: 30 s at 60 °C) was performed (Takara, #RR820A) on a CFX96 or CFX384 Real-Time System (Bio-Rad) using specific primer pairs. ..

    Article Title: Klebsiella pneumoniae T6SS exacerbates gut inflammation promoting tumorigenesis
    Article Snippet: .. RNA was extracted using Aurum total RNA kit (Bio-Rad), and cDNA was synthesized using iScript cDNA synthesis kits (Bio-Rad). qPCR was performed with SsoAdvanced Universal SYBRGreen SuperMix (Bio-Rad) on a CFX384 Real-Time System (Bio-Rad). qPCR primers are detailed in the Key Resource Table. ..

    Article Title: Liver steatosis induced by per- and polyfluoroalkyl substances exerts a limited influence on heterocyclic aromatic amine-mediated DNA damage at population-relevant exposure levels.
    Article Snippet: RT-qPCR analysis 202 cDNA was synthesized from extracted RNA using the High-Capacity cDNA Reverse Transcription Kit 203 (Applied Biosystems, Thermo Fischer Scientific, Waltham, MA, USA). .. Quantitative PCR was carried out, on 1 204 ng equivalent mRNA, using the CFX384 Real-Time System (Bio-Rad, Hercules, CA, USA) with SYBR Green 205 chemistry (Applied Biosystems, Thermo Fischer Scientific), following the manufacturer’s instructions. ..

    Synthesized:

    Article Title: Klebsiella pneumoniae T6SS exacerbates gut inflammation promoting tumorigenesis
    Article Snippet: .. RNA was extracted using Aurum total RNA kit (Bio-Rad), and cDNA was synthesized using iScript cDNA synthesis kits (Bio-Rad). qPCR was performed with SsoAdvanced Universal SYBRGreen SuperMix (Bio-Rad) on a CFX384 Real-Time System (Bio-Rad). qPCR primers are detailed in the Key Resource Table. ..

    cDNA Synthesis:

    Article Title: Klebsiella pneumoniae T6SS exacerbates gut inflammation promoting tumorigenesis
    Article Snippet: .. RNA was extracted using Aurum total RNA kit (Bio-Rad), and cDNA was synthesized using iScript cDNA synthesis kits (Bio-Rad). qPCR was performed with SsoAdvanced Universal SYBRGreen SuperMix (Bio-Rad) on a CFX384 Real-Time System (Bio-Rad). qPCR primers are detailed in the Key Resource Table. ..



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